Long-distance transport of Gibberellic Acid Insensitive mRNA in Nicotiana benthamiana
نویسندگان
چکیده
منابع مشابه
Replication-independent long-distance trafficking by viral RNAs in Nicotiana benthamiana.
Viruses with separately encapsidated genomes could have their genomes introduced into different leaves of a plant, thus necessitating long-distance trafficking of the viral RNAs for successful infection. To examine this possibility, individual or combinations of genome segments from the tripartite Brome mosaic virus (BMV) were transiently expressed in leaves of Nicotiana benthamiana plants usin...
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Small interfering RNAs can induce RNA silencing throughout plants owing to their ability to move through the phloem. Although microRNA (miRNA), another noncoding small RNA that regulates protein-coding RNAs through transcript cleavage or translational inhibition, has also been detected in phloem, there are few reports of its long-distance movement via phloem. We investigated whether miR172 mole...
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Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...
متن کاملOverexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance.
Aluminium (Al) toxicity is the most important limiting factor for crop production in acid soil environments worldwide. In some plant species, application of magnesium (Mg(2+)) can alleviate Al toxicity. However, it remains unknown whether overexpression of magnesium transport proteins can improve Al tolerance. Here, the role of AtMGT1, a member of the Arabidopsis magnesium transport family invo...
متن کاملMonoclonal Antibody Purification (Nicotiana benthamiana Plants).
Plant-based expression systems provide an alternative biomanufacturing platform for recombinant proteins (Matoba et al., 2011). In particular, plant virus-based vectors can overexpress proteins within days in the leaf tissue of Nicotiana benthamiana (N. benthamiana). To overcome the issues of genetic instability and limited infectivity of recombinant viruses, Agrobacterium-mediated delivery of ...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2013
ISSN: 1471-2229
DOI: 10.1186/1471-2229-13-165